Digital Biology Home > Manufactured Products > Other Products > MS-100

Micro Smash MS-100

Disruption and emulsion of a wide range of organisms inculuding bacteria, animal and plant tissues, yeasts, algae, and fungi
Disruption and emulsion of environmental samples such as soil and paint

Switch to Japanese Switch to Chinese

Line UP

Micro Smash MS-100

Key Features

  1. Patent applying "3D Rotating High Speed Motion"* for quick disruption of those fibrous tissues and resistant cells
  2. Five patterned memory settings and user optional condition settings
  3. A wide renge of rotation speed settings from 2,000 to 5,500 rpm
  4. Brushless motor realizing no brush particle generation and no brush replacement
  5. Easy to remove sample tube holder
  6. Easy to fix sample tubes with the turn of the central knob
  7. Easy to monitor the disruption process through the clear lid
  8. Economical and compact tabletop system, accommodating up to 2.0ml×12 tubes
  9. Processing up to 6×5ml tube with an additional tube holder (optional)

3D Rotating High Speed Motion

"3D Rotating High Speed Motion" is a high-speed movement which vertically rotates and moves the sample tubes in a "figure-eight" motion. This motion allows beads to impact the cells more directly for effective disruption.

Sample animated images

Sample animated images of the "3D High Speed Oscillating movement" can be viewed by pressing the "STOP" button/"STERT" button.

DNA extraction from yeast cells; S. pombe
Proteinase K Method vs Beads Cell Disrupter MS-100

Methods

  1. In preparation for examination, yeasts collected from the culture broth at an OD660 of 0.20 were mixed with buffer.
  2. Perform following three methods for cell disruption, two processes each;
    a. Processed without beads (as a negative control)
    b. Processed with the Proteinase K Method
    c. Processed with MS-100, using 1mm glass beads at 3,000rpm for 5 minutes
  3. After disruption, each sample was phenol extracted, ethanol precipitated and electrophoresed on a 1% agarose gel.

ph

Genomic DNA and RNA, which were hardly extracted by using Proteinase K method, could be successfully obtained with MS-100.

RNA extraction from rice (Oryza sativa L.) leaves (fresh leaves)
Using a commercially available DNA extraction kit to obtain RNA

Beads and Disruption Conditions

  1. φ4.8 stainless steel beads x 2
    at 3,500rpm for 90 sec x 2
  2. φ5.5 stainless steel beads x 1
    at 3,500rpm for 90 sec x 2
  3. φ3.2 stainless steel beads x 10
    at 3,500rpm for 120 sec
  4. φ4.8 stainless steel beads x 2
    at 4,000rpm for 120 sec
  5. φ5.5 stainless steel beads x 1
    at 4,000rpm for 120 sec
  6. φ5 zirconium beads x 1
    at 4,300rpm for 100 sec

ph

MS-100 reduces the amount of low molecular weight RNA and allows recovery of high quality RNA.

Methods

  1. Add 450μl of Buffer to a 2ml tube containing a specified amount of beads.
  2. Put 80 to 90mg rice leaves into the tube.
  3. Disrupt the sample using the TOMY Beads Cell Disrupter MS-100.
  4. Perform extraction and purification according to the procedure given to the kit.
  5. After measuring A260 value of the recovered RNA solution, 5μg of total RNA of each sample was subjected to electrophoresis through a 1.2% denatured agarose gel.

Reference Data

Special Order Items

ph
  1. 2ml×12 tube holder with loop cap P-00102
  2. 5ml×6 tube holder P-00103
  3. Auxiliary tool for fastening the central knob (power knob) P-00110

Specifications

ModelMS-100
Speed Setting Range2,000 to 5,500rpm/100rpm increment
Disruption MethodMicroprocessor controlled "3D Rotating High Speed Motion" (patent applying)
Drive MotorBrushless, induction drive motor
Timer Setting Range1 to 300 seconds or 1 to 100 seconds more then 5,100rpm
DisplayLCD digital display with backlight
Capacity2ml x 12tubes
Safety DevicesLid Opening Detector, Motor Overheat Detector and Rotor Malfunction Detector
Dimensions280W x 320D x 305Hmm
Net weightApprox.17kg
Power InputAC120V, 3A, 50/60Hz, 1P
AC220V/230V/240V, 2A, 50/60Hz, 1P
Accessories Includedφ0.1/φ0.5/φ1.0 Glass Beads and φ2.0 Zirconia Beads,
approx. 30g each and 2ml x 20 sample tubes

* Specifications and design are subject to change for improvement without prior notice. Please ask for the latest specifications prior to your order and procurement.

See Catalog PDF for details.

* This document is created in the PDF file format.
* To view PDF files, Adobe Reader or other software is needed.
* If you don’t have Adobe Reader, click here to download its latest version.
PDF downroad MS-100 (1.6MB)
TOMY Other and related products