ゲノミクスの進歩は、遺伝子制御に関する我々の理解を一変させ、発生生物学から疾患研究に至るまで、様々な分野における画期的な発見に道を開きました。この変革の最前線にあるのが、遺伝子制御の複雑さを解明するためにデザインされたゲノミクスツール、Dovetail Pan Promoter Panelです。
本セミナーでは、Dovetail Pan Promoter Panelのパワーを探るべく、制御ゲノミクスの世界への旅に皆様をご招待します。このセミナーでは、この分野の著名な専門家を講師に迎え、パネルの比類ない能力と、遺伝子発現の隠された秘密を解き明かすその可能性について掘り下げていきます。
Speaker 1: Professor Yasuhiro Murakawa
Kyoto University Institute for Advanced Study (KUIAS)
Dissecting human disease pathways using high-resolution chromatin contact maps
Large-scale genome-wide association studies (GWAS) have yielded an increasing number of disease-associated genomic loci. However, the functional interpretation still largely remains unclear. Recently, it has become apparent that disease-associated genetic variants are often found within enhancers. Enhances act to strongly enhance the expression of their target genes in a cell-type specific fashion, by physically associating with their promoters. We have developed a 5’-end single-cell RNA sequencing approach to comprehensively map active enhancers from heterogeneous helper T cells. By integrating with GWAS datasets, we identified hundreds of human enhancers associated with autoimmune diseases. To gain important clues to human disease pathways, here we used Micro-C as well as promoter-capture Micro-C, methods that can analyze chromatin interactions with super-high resolution. We systematically identified target genes of these enhancers, revealing novel human disease molecular mechanisms. In sum, we provide a general framework to investigate molecular mechanisms underlying human diseases.
Speaker 2: Myriam Elkhawand
Customer Success Manager, Dovetail Genomics
Setting up the Dovetail® Pan Promoter Assay in the lab
In this second talk, Myriam will delve into the details of Dovetail Pan Promoter protocol and library quality control measures that play a pivotal role in assessing the success of the assay. She will discuss best practices in carry out the assay, library QC, emphasizing the importance of stringent quality standards for reliable data analysis. She will also share the analysis pipeline for Dovetail Pan Promoter panels.
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